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TIDES USA 2022 | Hzymes Product Profile: recombinant Trypsin & Trypsin Assay Kit (ELISA)

The 2022 Biopharmaceutical Conference Tides USA will be launched soon. Hzymes will bring bio-fermentation products and quality control solutions such as recombinant trypsin and trypsin residue detection kits, to Boston to better help accelerate the R&D and production of biopharmaceuticals.

                                                                                                                              Recombinant Trypsin

With the rapid development of the world’s biopharmaceutical field, research on monoclonal antibodies, recombinant proteins, vaccines, and drugs related to gene and cell therapy has received great attention. The fundamental element of most biopharmaceutical active substances is cells. From the acquisition of primary cells in the early stage to the final large-scale cell factory or microcarrier reactor culture, trypsin and trypsin analogs are often used for multiple digestion and passage of cells, and the digestion effect of trypsin will significantly affect the drug activity: substance production and downstream applications.

Trypsin was initially discovered and used in the pancreas of mammals, including many species such as cattle, sheep, and pigs. Because trypsin derived from mammalian cells is most similar to the human body, it is widely used in cell culture. However, different sources of trypsin have different optimal temperatures. Moreover, there is a huge danger in introducing animal-derived trypsin in the production process of modern biopharmaceuticals. Trypsin obtained by natural extraction may contain a variety of zoonotic diseases in animals, such as foot-and-mouth disease virus, prion, influenza virus, etc. Contaminate biological products.

The recombinant porcine trypsin of Hzymes can be produced on a large scale by using Escherichia coli or Pichia pastoris as the expression host, effectively avoiding animal-derived pollution and having higher biological safety. This recombinant trypsin can effectively replace traditional natural pancreatin in biopharmaceutical fields such as insulin production and inactivated vaccines.


Product advantages:

No Animal Origin: Recombinant trypsin is free from exogenous virus contamination. Trypsin extracted from porcine and bovine pancreas has the risk of viral infection. It is necessary to detect the content of viruses (such as porcine parvovirus, etc.) in nature trypsin.

Process stability: The recombinant trypsin process is stable, with little difference between batches and batches.

High activity: According to the test, the specific activity of recombinant trypsin is about five times higher than animal-extracted trypsin.

Digestion termination: Recombinant trypsin can be directly diluted with PBS to terminate digestion in the laboratory. Soybean trypsin inhibitor can be added to stop digestion during large-scale production to avoid fatal changes caused by complex components in serum.

High yield: Recombinant trypsin is expressed through recombinant E.coli or Pichia pastoris cells, which have access to mass production.

                                                                                                                    Trypsin Assay Kit (ELISA)

                                                                                    Rapid and Accurate Detection of Pancreatic Enzyme Residues

In developing and producing vaccines and viral vectors, culturing adherent cells is an important part. Trypsin is usually used to digest and disperse cells and collect seed cells. When digesting cells, the amount of trypsin used and the digestion time should be strictly controlled, and the digestion reaction should be terminated after the cells are digested. Excess trypsin residues can affect the downstream purification of the drug product and the purity of the vaccine stock solution.

In response to this demand, Hzymes has launched a trypsin detection kit (ELISA method), which is specially designed to detect trypsin residues in samples. The kit is based on a double-antibody sandwich enzyme-linked immunosorbent assay. The detection range (0.039-5ng/mL) and limit of quantification (0.003 ng/mL) meet drug production’s detection needs and better assist biopharmaceutical-related customers in quality control.


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