Can Isothermal Amplification Technology counterattack In The Molecular POCT Market?

SARS-CoV-2 epidemic that began in 2020 has greatly promoted the development of the global molecular diagnostics market and technology. In the field of professional diagnosis, qPCR, with its advantages of high sensitivity, high specificity, high throughput, and high automation, has shined in the epidemic prevention and control, and is recognized as the gold standard for pathogen diagnosis!

molecular POCT
  1. Molecular POCT application scenarios

With the continuous spread of SARS-CoV-2 epidemic, molecular diagnostic technology is being developing, and its application scenarios are gradually diversifying. With the characteristics of fast, convenient, high sensitivity, able to go out of the professional laboratory, demand for user-centric molecular POCT is rapidly increasing worldwide, and so its market has been further opened up– molecular diagnosis has moved further into the home self-test market. The era of molecular POCT has truly arrived!

  1. Brief Analysis of Molecular POCT Application Scenarios

In different application scenarios, different technical routes have their own advantages and disadvantages, so each technical route has its own unique applicable scenarios.

Brief Analysis

2.1Scenario requirements comparison

2.2Comparison of technical features

2.3 From point-of-care testing to customs fast inspection and self-inspection at home, the molecular POCT market continues to explode

Molecular POCT has a wide range of application scenarios, and the detection demand is always there! It’s just that qPCR has not been able to be effectively satisfied due to the high cost of instruments, site and personnel before!

molecular POCT market

Point-of-care testing

Isothermal and quick inspection

Self-inspection at home

After the outbreak of SARS-CoV-2, instruments and reagent technology developed rapidly in China, and many products were launched, which greatly promoted the development of the molecular POCT market.

  • The key of isothermal amplification technology in molecular POCT counterattack

With the advantages of fast detection speed and convenient operation, isothermal amplification technology perfectly matches the molecular POCT market!

The module of the isothermal amplification instrument is simple. After further canceling the use of the computer screen, the change from interpreting the CT curve results to the signal light directly indicating the negative and positive results makes the molecular POCT truly enter the self-inspection home market, which is the biggest success of the isothermal amplification technology!

Improving the sensitivity and specificity of isothermal amplification technology and reducing the cost of a single reagent are the key to the constant development of isothermal amplification technology in the field of molecular POCT and the continuous development of molecular POCT.

After years of technical development and accumulation of experience, Hzymes biotech has carried out in-depth research and optimization on the sensitivity and specificity of the system, starting from isothermal amplification core enzyme raw materials, and has tackled one by one difficult problems such as freeze-drying process, industrial quality control and cost optimization etc..Hzymes Launched a full range of isothermal amplification technology solutions to help partners to expand the molecule POCT market together and serve a wider range of user needs!

  1. Hzymes SFAC-LAMPTechnology Platform

3.1 Introduction to SFAC-LAMP Technology Platform

Introduction to SFAC-LAMP Technology Platform

3.2 Fast Amplification

3 batches of tests, peaking in 8 minutes on average

3.2 Fast Amplification 3 batches of tests, peaking in 8 minutes on average

Number of cycles 101Template RT-LAMP peak profile

3.3 High sensitivity

Number of cycles 40copies/T

3.3 High sensitivity

Number of cycles 20copies/T

3.4 High specificity

NTC test, 0/48;

3.4 High specificity NTC test, 0/48;

Number of cycles

3.5 Good thermal stability

Green: 1 day at 37°C; Red: 7 days at 37°C

3.5 Good thermal stability Green: 1 day at 37°C; Red: 7 days at 37°C

Number of cycles

3.6 SFAC-LAMP primer optimization

It is complex and difficult to design the primer of LAMP system. The quality of primers directly determines the performance of the amplification system. Based on years of research on the application of LAMP in SARS-CoV-2, ASF, A-B and other applications, Hzymes Biotech has launched primer design services and finished primers for popular pathogens to assist users in quickly developing products for certification and listing.

3.6 SFAC-LAMP primer optimization It is complex and difficult to design the primer of LAMP system. The quality of primers directly determines the performance of the amplification system. Based on years of research on the application of LAMP in SARS-CoV-2, ASF, A-B and other applications, Hzymes Biotech has launched primer design services and finished primers for popular pathogens to assist users in quickly developing products for certification and listing.

Primer optimization service case:

By adjusting the concentration, position of nucleotide base pairs, the distance between DNA regions and Tm value, etc., the peak time is greatly advanced.

Primer optimization service case: By adjusting the concentration, position of nucleotide base pairs, the distance between DNA regions and Tm value, etc., the peak time is greatly advanced.

3.7 Lyophilization performance

Red: liquid dosage form test;

green: tube bottom freeze-dried dosage form test;

Freeze-dried ball performance test

Number of cycles

Number of cycles

Number of cycles

Number of cycles

  1. Hzymes SFAC-LAMP overall solution
    • HzymesSFAC-LAMP overall solution product table
  • Nucleic Acid Amplification

Lysis buffer for direct amplification

  • Primer study

Primer design

Primer optimization

SARS-CoV-2 primer set

monkeypox primer set

  • Core Raw Material

Bst DNA Polymerase V1

Bst DNA Polymerase V2

Bst DNA Polymerase V2 (High concentration)

RTL reverse transcriptase

RTL reverse transcriptase(High concentration)

RNase Inhibitor

dNTP series

  • Reagent

RT-LAMP Colorimetric Master Mix (Red/Yellow)

master mix (liquid), ready to lyophilize master mix, in situ lyophilization, lyophilized pellet.

RT-LAMP Fluorescent Master Mix

master mix(liquid), ready to lyophilize master mix, in situ lyophilization, lyophilized pellet.

 

  • CRO&OEM

Primer Optimization Design Service

Lyophilization Process Development Service

Lyophilization OEM Service

SARS-CoV-2 Assay Kit OEM Service

The recommended way of cooperation: Hzymes OEM solution

Compared with self-developed by users, Hzymes OEM solution has the following advantages.

Catalog

Contact us to get a sample test:

info@hzymes.com 

021-60879177

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