CACLP Products List | Talk about molecular diagnostics

Molecular diagnosis, as the name suggests, is a professional process that detects various samples through molecular biology methods to provide microscopic basis for disease diagnosis and treatment, infection and epidemic prevention, and forensic identification. In this process, it is inseparable from the discovery of core raw materials for molecular diagnosis and the invention of important technologies.

Dr. Kary Mullis was primarily responsible for the synthesis of oligonucleotides (small pieces of DNA) during his tenure as Head of Synthesis at Cetus Biologics.

In 1983, when Mullis was driving down the highway, he had in mind the structure of the double strands of DNA and how to keep DNA fragments replicating themselves.

In November 1984, he led several technicians to successfully complete the first PCR experiment, performing 10 PCR cycles of replication amplification of a 49 bp DNA fragment.   

With the development of molecular biology technology, molecular diagnostics include PCR (qPCR and dPCR), second-generation sequencing technology (NGS), fluorescence in situ hybridization (FISH) and gene chips and other technology platforms with rapid development, and the use scenarios have also developed from genetic research, infectious disease diagnosis to early diagnosis of tumors, accompanying diagnosis, precision medicine and other frontier fields.

(1) PCR

Principle: DNA forms a single strand at high temperatures, and double-strands are formed at low temperatures according to the principle of base complementary pairing

Pros and cons: high sensitivity, strong specificity, simple and fast, but a single detection site, only known mutations can be detected.

Main application areas: infectious diseases, early diagnosis of tumors, genetic diseases

(2) FISH

Principle: Using the nucleic acid of the known sequence of fluorescence-specific labeling as a probe, hybridization with the nucleic acid in the cell or tissue section to accurately and quantitatively locate the specific nucleic acid sequence.

Pros and cons: high sensitivity, strong specificity, can be detected in situ on the tissue, but the detection cost is high; The operation is cumbersome, time-consuming and subjective.

Main application areas: virus detection, solid tumors, hematological tumors.

(3) NGS

Principle: Through the chemical modification of the template DNA molecule, it is anchored on the nanopores or microcarrier chips, and the interpretation of the base sequence is realized by collecting fluorescent labeling signals or chemical reaction signals by using the principle of base complementary pairing.

Pros and cons: large flux, high sensitivity, can detect a variety of mutations, but the test operation is complex, high cost, there is a certain inherent error rate.

Main application areas: early tumor screening, genomics, non-invasive prenatal diagnosis, clinical research

(4) Gene chips

Principle: Hybridization sequencing – a probe that fixes a sequenced known target nucleotide on the surface of a gene to determine the complementary matching of the sequence

Pros and cons: high flux, high sensitivity, high accuracy, but difficult to develop, low flexibility, small detection flux, only known genes can be detected

Main application areas: drug screening, new drug development, disease diagnosis

As a comprehensive supplier of core raw materials for molecular diagnosis, Hzymes Biotech has accumulated a large number of excellent products in the rapid PCR detection technology platform of molecular diagnosis, the LAMP isothermal amplification technology platform, and the mNGS sequencing technology platform, and at the same time, on the basis of the combination of products and technical services, it provides comprehensive support for Chinese head molecular diagnostic enterprises.

The main molecular diagnostic products of Hzymes Biotech are: