CACLP Products List | Talk about molecular diagnostics

It is estimated that the term “nucleic acid detection” is already known to everyone, and there are rap lyrics written specifically for “doing nucleic acid”, which can be called a brainwashing song. But what is “nucleic acid detection”? I believe everyone has some understanding from various scientific articles. The official name of “nucleic acid detection” in the field of in vitro diagnosis can be called molecular diagnosis.Today we will talk about “molecular diagnosis” from the perspective of the development history of “nucleic acid detection” and raw materials!

Hzymes Molecular diagnostic

Molecular Diagnostics

Molecular diagnosis, as the name suggests, is a professional process that detects various samples through molecular biology methods to provide microscopic basis for disease diagnosis and treatment, infection and epidemic prevention, and forensic identification. In this process, it is inseparable from the discovery of core raw materials for molecular diagnosis and the invention of important technologies.

Dr. Kary Mullis was primarily responsible for the synthesis of oligonucleotides (small pieces of DNA) during his tenure as Head of Synthesis at Cetus Biologics.

In 1983, when Mullis was driving down the highway, he had in mind the structure of the double strands of DNA and how to keep DNA fragments replicating themselves.

In November 1984, he led several technicians to successfully complete the first PCR experiment, performing 10 PCR cycles of replication amplification of a 49 bp DNA fragment.   

Molecular article image 1

In 1988, the heat-resistant DNA polymerase was extracted from an aquatic thermophagocytobacillus (thermus aquaticus) isolated from a hot spring by Saiki et al., and then introduced into PCR technology, which greatly simplified the PCR reaction process and made its application in the clinic possible.

In 1989, the journal Science reported on PCR technology and high-temperature-resistant DNA polymerase, and Cetus also cooperated with Roche to use PCR technology for clinical diagnosis.

In 1991, Roche Pharmaceutical bought Cetus’ patent for PCR technology for $300 million.

In 1993, the jury decided to award the Nobel Prize in Chemistry to Mullis for the development of the polymerase chain reaction method (PCR).

With the development of molecular biology technology, molecular diagnostics include PCR (qPCR and dPCR), second-generation sequencing technology (NGS), fluorescence in situ hybridization (FISH) and gene chips and other technology platforms with rapid development, and the use scenarios have also developed from genetic research, infectious disease diagnosis to early diagnosis of tumors, accompanying diagnosis, precision medicine and other frontier fields.

(1) PCR

Principle: DNA forms a single strand at high temperatures, and double-strands are formed at low temperatures according to the principle of base complementary pairing

Pros and cons: high sensitivity, strong specificity, simple and fast, but a single detection site, only known mutations can be detected.

Main application areas: infectious diseases, early diagnosis of tumors, genetic diseases

(2) FISH

Principle: Using the nucleic acid of the known sequence of fluorescence-specific labeling as a probe, hybridization with the nucleic acid in the cell or tissue section to accurately and quantitatively locate the specific nucleic acid sequence.

Pros and cons: high sensitivity, strong specificity, can be detected in situ on the tissue, but the detection cost is high; The operation is cumbersome, time-consuming and subjective.

Main application areas: virus detection, solid tumors, hematological tumors.

(3) NGS

Principle: Through the chemical modification of the template DNA molecule, it is anchored on the nanopores or microcarrier chips, and the interpretation of the base sequence is realized by collecting fluorescent labeling signals or chemical reaction signals by using the principle of base complementary pairing.

Pros and cons: large flux, high sensitivity, can detect a variety of mutations, but the test operation is complex, high cost, there is a certain inherent error rate.

Main application areas: early tumor screening, genomics, non-invasive prenatal diagnosis, clinical research

(4) Gene chips

Principle: Hybridization sequencing – a probe that fixes a sequenced known target nucleotide on the surface of a gene to determine the complementary matching of the sequence

Pros and cons: high flux, high sensitivity, high accuracy, but difficult to develop, low flexibility, small detection flux, only known genes can be detected

Main application areas: drug screening, new drug development, disease diagnosis

As a comprehensive supplier of core raw materials for molecular diagnosis, Hzymes Biotech has accumulated a large number of excellent products in the rapid PCR detection technology platform of molecular diagnosis, the LAMP isothermal amplification technology platform, and the mNGS sequencing technology platform, and at the same time, on the basis of the combination of products and technical services, it provides comprehensive support for Chinese head molecular diagnostic enterprises.

The main molecular diagnostic products of Hzymes Biotech are:

molecular diagnostic products

Highly active, more stable proteinase K

Let the world use China’s high-end proteinase K

【Product Advantages】

  • Enzyme directed evolution engineering acquisition, higher stability, higher enzyme activity
  • Excellent ability to tolerate guanidine salts
  • No heteroenzyme residue, nucleic acid residue < 5 pg/mg, no heavy metal ions
  • With an annual output of 1500 kg, it is the largest supplier of proteinase K in China
    【Test data】
Proteinase K
PRK test Image

【Ordering Information】

【prk Ordering Information】

Nucleic acid extraction solutions 

6.5 min Sample processing is done in lightning speed

【Product Advantages】

  • The whole extraction process is completed in 6.5 minutes, providing lightning speed for molecular POCT detection
  • The system is robust and compatible with up to 400 ul sample throughput
    The proposed high efficiency and minimum 10copies can ensure the extraction effect and reduce the false negative rate of sample detection

Available in pre-assembled and OEM bulk packages
【Test data】

Nucleic acid extraction solutions test 2

【Ordering Information】

【Ordering Information】 Nucleic

Ultra-stability Taq DNA Polymerase

【Product Advantages】

  • Long-range PCR to amplify products up to 5 kb
  • High PCR sensitivity with the minimum detection of 0.1 pg DNA

Equipped with Class 10000 GMP workshops, a fermentation production line with a maximum productivity of 10 tons/d, and an automatic packaging line
【Test data】

【Ordering Information】

【Ordering Information】ultra

Super closed hot-start Taq Polymerase

【Product Advantages】

  • The enzyme is completely enclosed and the activity can be released after heating at 95 °C for 10 min
  • High sensitivity, detection concentrations as low as 0.1 pg
  • Available in Master Mix reagent form for ease of use
  • Provide compatible UDG/dUTP anti-pollution system Master Mix
  • Strong compatibility, suitable for multiple PCR system
    【Test data】

Chemically modified enzyme activity blocking assay

Hot-start Taq DNA polymerase (chemical modification) of Hzymes Biotech is completely blocked after chemical modification, and the activity can be released after heating at 95℃ for 10min.

HPV typing test

Hzymes Hot-start Taq DNA polymerase (chemical modification)  has good compatibility and is suitable for various PCR systems.

Sensitivity detection

Sensitivity detection

Hzymes Hot-start Taq DNA polymerase (chemical modification)  has high sensitivity and can detect concentrations as low as 0.1pg

【Ordering Information】

【Ordering Information】

Ultra-sensitive hot-start Taq Polymerase

【Product Advantages】

  • Can be activated quickly, 95 °C 2min activation enzyme activity
  • High sensitivity, detection concentrations as low as 0.1 pg
  • Provide compatible UDG/dUTP anti-pollution system Master Mix
  • Applicable to multiple PCR systems
  • Strong adaptability, compatible with dye method and probe method qPCR
  • Strong stress resistance, can resist 10% whole blood
    【Test data】
Ultra-sensitive hot-start Taq Polymerase

Antibody modified enzyme activity blocking detection

Hot-start Taq DNA polymerase (antibody modification) of Hzymes Biotech is completely blocked after antibody modification, and the activity can be released after heating at 95℃ for 2min.

Hzymes Hot-start Taq DNA polymerase (antibody modification)

 Hzymes Hot-start Taq DNA polymerase (antibody modification)  has better sensitivity than foreign competitors.

Sensitivity detection

Sensitivity detection Hzymes Hot-start Taq DNA polymerase (antibody modification) has high sensitivity and can detect concentrations as low as 0.1pg

【Ordering Information】

Reverse transcription solution

Reverse transcriptase                                                               

Product Advantages】

  • The temperature adaptation range is wide, and the reverse transcription reaction can be completed at 37-60 °C
  • High purity, no nuclease contamination, sterile DNA residues
  • Good compatibility, can amplify high GC template and complex advanced structure template
  • It can transcribe long fragments of RNA up to 10 kb
  • A glycerin-free version is available to meet lyophilized needs

【Performance data】

【Performance data】

【Product Information】

RNase inhibitors

【Product Advantages】

  • Extensive inhibition of various RNase(B,C,A)
  • No nuclease contamination, sterile DNA residue

【Performance data】

【Product Information】

【Product Information】

Deoxyribonucleoside triphosphate (dNTP)

High purity dNTP for nucleic acid detection

【Product Advantages】

  • The purity of dNTP HPLC is higher than 99%, which ensures the amplification efficiency
  • no Dnase, Rnase pollution, no heavy metal ion residue, to ensure detection sensitivity

no source, bacterial DNA, to ensure no background interference
【Test data】

Deoxyribonucleoside

【Ordering Information】

Due to the needs of Chinese epidemic prevention and control, molecular diagnostic laboratories have achieved popularity in hospitals at all levels, and the demand for molecular diagnostic technology platforms and related products is also increasing day by day, while different application scenarios have different requirements for the products applicability. Hzymes Biotech  launches of different technology platform molecular diagnostic products for different application scenarios.

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