African swine fever virus detection solution (ASFV)
African swine fever is a highly contagious and severe infectious disease caused by the African swine fever virus (ASFV), which can infect both domestic pigs and wild boars. The disease has a mortality rate of up to 100% and poses a serious threat to the global pig industry, causing significant economic losses.
Currently, there is no vaccine or effective treatment available for African swine fever. The qPCR method is a recommended tool for routine diagnosis of the disease by the World Organisation for Animal Health (OIE), and it is also the preferred detection technology in China during the ongoing epidemic.
Hzymes biotech, a supplier of enzyme preparations in the upstream life science field, has been providing high-quality and cost-effective raw materials, including freeze-dried materials, to in vitro diagnostic (IVD) companies for a long time. In response to the African swine fever epidemic, Hzymes biotech is collaborating with its customers to develop a complete process for the detection of the ASFV, from extraction to detection.
This solution offers an efficient way to detect African swine fever and can help mitigate the impact of the disease.
African swine fever virus (ASFV) detection solution
Nucleic Acid Extraction & qPCR Solutions
- Magnetic Bead Method Nucleic Acid Extraction Kit
- Super strong premixed stability qPCR reagent
Product | Product Description | Product Name | Cat. No |
Nucleic acid extraction (magnetic bead method)
| bottled form 32-channel pre-packaged form 96-channel pre-packaged form
| ASFV Magnetic Swab Viral DNA/RNA Kit ASFV Mag32 Swab Viral DNA/RNA Kit ASFV Mag96 Swab Viral DNA/RNA Kit | HMD8001 HMD8002 HMD8003
|
Nucleic acid amplification testing
| l Fully premixed stability optimized version (including UDG) l Freeze-dryable kits
| ASFV TaqMan Multiplex qPCR Master Mix(UDG plus) ASFV Lyo-TaqMan Multiplex qPCR Kit
| HMD8004 HMD8005 |
ASFV Detection Solution: Nucleic Acid Extraction & qPCR
Viral DNA/RNA Extraction Kit from Magnetic Beads Swab Sample + Super Full Premixed Stable qPCR Reagent
ASFV Magnetic Swab Viral DNA/RNA Kit
The product is primarily designed for the isolation and purification of high-quality viral DNA/RNA from a variety of swabs and virus culture supernatants. It utilizes magnetic beads with a unique separation effect, in combination with a well-optimized buffer system, to efficiently capture the released nucleic acid. As a result, the extracted nucleic acid has a high yield and excellent quality, making it suitable for various downstream applications, such as PCR and fluorescent quantitative PCR.
Product advantages
- Excellent performance: high extraction efficiency and good repeatability
- Safe and non-toxic: the reagent does not contain toxic substances such as phenol and chloroform
ASFV TaqMan Multiplex qPCR Master Mix(UDG plus)
The product includes a hot-start Taq enzyme that is blocked by double-blocking antibodies, effectively inhibiting both its 5’→3′ polymerase and 5’→3′ exonuclease activities. This double-blocking mechanism significantly enhances the amplification efficiency, specificity, and stability of the reagents. Additionally, the product features a dUTP/UDG anti-pollution system that prevents aerosol pollution, ensuring a high level of experimental accuracy and reliability.
Product advantages
High amplification efficiency
Super full premix stability
Anti-pollution system
Data Display
Stability: The qPCR master mix is prepared as a full master mix containing primers and probes, placed at 37°C for 7 days, 4°C for 9 days, repeated freezing and thawing 20 times, the amplification is high, There was no significant change in the Ct value of medium and low concentrations of ASFV plasmid.
Comparison of competing products: take African swine fever-positive blood samples and perform 10-fold serial dilution, the results show good linearity (R > 0.99), fluorescence curve and fluorescence
Values are higher than competing products.
Data
High sensitivity 5 copies/reaction: repeat the amplification concentration 5 copies/reaction template 20 times, the detection rate is over 95%.
Linear range 10-10 copies/reaction: 10-fold isocratic dilution of template samples, 10-10 copies/reaction concentration range is linear with a wider lines range.
Hzymes biotech ASFV detection single enzyme raw material supply
In addition to the previously mentioned solutions, Hzymes biotech also offers a range of single-product components at a cost-effective price point. These include proteinase K, hot-start Taq polymerase with chemical modification, hot-start Taq polymerase with antibody modification, and Taq DNA polymerase, among others. These components, including lyophilized raw materials, are designed to meet the diverse needs of in vitro diagnostic (IVD) enterprises.
Product Type | Product Name | Cat. No. |
Single Enzyme Raw Material
| Proteinase k | HH4500 |
Hot Start Taq Polymerase (Chemical Modification) | HMD0201
| |
Hot Start Taq Polymerase (Antibody Modification) | HMD0202
| |
Taq DNA polymerase | HMD010 |