Hzymes Biotech
LAMP/RT-LAMP - RAPID NUCLEIC ACID DETECTION
Loop-mediated isothermal amplifification (LAMP) is a versatile technique for detecting target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. LAMP technique does not require changing the reaction temperature and it’s extremely fast, which is making them particularly well suited for field applications and point-of-care molecular diagnostics assays.
With well-designed 4-6 primers and the specifific Bst DNA polymerase, the detection of the target of interest can be done within 30 min. There are many LAMP detection methods, including the flfluorescent method, colorimetric method, fluorescent-probe-based method, turbidity method, HNB, etc. The LAMP solution provided by Hzymes is able to help achieve high sensitivity, good primer compatibility, and excellent performance in specifificity, tolerance, and stability.
OUR ADVANTAGES
BST DNA POLYMERASE V2
Fast polymerization
A fast polymerization is the key of Bst DNA Polymerase, the Bst DNA Polymerase V2 (HMD7003) performs almost the same speed compared against the competitor with the SARS-COV-2 N gene target. And better after 7 days thermal acceleration experiment.
Warmstart activity
To achieve higher specifificity in the LAMP reaction and even close to zero false-positive amplifification, Bst DNA Polymerase V2 (HMD7003) with a reversibly-bound aptamer could give you the warmstart function, which can block the activity under room temperature. Hzymes Bst DNA Polymerase V2 could have higher blocking efficiency at 25℃, 37℃, and 40 ℃, and can release the activity even faster at 65 ℃ than other competitors.
High Tolerance to Salt Inhibition
LAMP reactions were performed using Bst DNA Polymerase V2 (HMD7003) or a competitor Bst Polymerase (Company N) using increasing amounts of salt (potassium chloride). Bst DNA Polymerase V2 from Hzymes has better overall performance in the presence of potassium chloride.
Long-Term Stability
The activity test of Bst DNA Polymerase V2 (HMD7003) for 12 months under proper storage conditions. It has no activity change even after 12 months of storage.
RT-LAMP MASTER MIX (FLUORESCENT) - LIQUID & LYOPHILIZED
High Sensitivity and High Inhibitor Tolerance
RT-LAMP reaction is performed using a nasal swab sample spiked with SARS-CoV-2 pseudovirus(50 copies/T, 100 copies/T, and 250 copies/T) after a simple treatment with Hzymes Sample Release Buffer(HMD3504).
High Specificity
There is no NTC amplifification while using RT-LAMP Master Mix(Fluorescent) (HMD5207).
Performance Consistency with Lyophilized Reagent
The data illustrate that the lyophilization process does not affect the performance of the RT-LAMP reaction. (The same template concentration: 250 copies/T, 100 copies/T, and 50 copies/T).
dUTP Tolerance
Hzymes RT-LAMP Master Mix(Fluorescent) (HMD5204) performs better dUTP tolerance than company N, while using the same primer set and template.
RT-LAMP MASTER MIX (COLOMETRIC) - LIQUID & LYOPHILIZED
The nasal swab samples spiked with SARS-CoV-2 pseudovirus (50 copies/T, 100 copies/T, 250 copies/T) can be 100% detected using RT-LAMP Master Mix (Colometric)(HMD5204). The samples have been simply treated using Hzymes Sample Release Buffer (LAMP Colometric)(HMD3505)
SELECTION GUIDE FOR YOUR LAMP APPLICATION
LAMP/RT-LAMP Raw Materials
Hzymes Cat. No. | Products | Specification |
HMD7002 | Bst DNA polymerase V2 | 800U, 8000U, 80000U |
HMD7002D | Bst DNA polymerase V2(High concentration) | 4800U, 12000U, 120000U |
HMD7003 | WarmStart Bst DNA polymerase V2 | 800U, 8000U, 80000U |
HMD5301 | RTL reverse transcription | 1500U, 15000U, 150000U |
HMD5301D | RTL reverse transcription(High concentration) | 9000U, 22500U, 225000U |
HMD3901 | RNase inhibitor | 2000U, 20000U, 400000U |
HMD2101 | dNTP(25mM each) | 0.5mL, 1mL, 5mL, 100mL |
HMD2102 | dNTP(10mM each) | 0.5mL, 1mL, 5mL, 100mL |
HMD3504 | Sample release buffer | 1mL, 8mL, 100mL, 1000mL |
HMD3505 | Sample release buffer(LAMP colometric) | 1mL, 8mL, 100mL, 1000mL |
LAMP/RT-LAMP related CDMO Service
Hzymes Cat. No. | Products |
HCRO0011 | LAMP primer design and optimization |
HCRO0012 | The development of lyophilization process |
HCRO0013 | Contract production of lyophilized reagent |
LAMP/RT-LAMP Master Mix
Hzymes Cat. No. | Products | Specification |
HMD5204 | LAMP/RT-LAMP colometic master mix (Liquid, Lyoph-Ready) | 100RXN, 1000RXN, 10000RXN |
HMD5205 | LAMP/RT-LAMP colometic master mix (In situ lyophilization) | 100RXN, 1000RXN, 10000RXN |
HMD5206 | LAMP/RT-LAMP colometic master mix (Lyophilized beads) | 100RXN, 1000RXN, 10000RXN |
HMD5207 | LAMP/RT-LAMP flfluorescent master mix (Liquid, Lyoph-Ready) | 100RXN, 1000RXN, 10000RXN |
HMD5208 | LAMP/RT-LAMP flfluorescent master mix (In situ lyophilization) | 100RXN, 1000RXN, 10000RXN |
HMD5209 | LAMP/RT-LAMP flfluorescent master mix (Lyophilized beads) | 100RXN, 1000RXN, 10000RXN |