Hzymes

"Hzymes" Each Molecule Promises Accuracy }

Hzymes Biotech

LAMP/RT-LAMP - RAPID NUCLEIC ACID DETECTION

Loop-mediated isothermal amplifification (LAMP) is a versatile technique for detecting target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. LAMP technique does not require changing the reaction temperature and it’s extremely fast, which is making them particularly well suited for field applications and point-of-care molecular diagnostics assays.

With well-designed 4-6 primers and the specifific Bst DNA polymerase, the detection of the target of interest can be done within 30 min. There are many LAMP detection methods, including the flfluorescent method, colorimetric method, fluorescent-probe-based method, turbidity method, HNB, etc. The LAMP solution provided by Hzymes is able to help achieve high sensitivity, good primer compatibility, and excellent performance in specifificity, tolerance, and stability.

Hzymes LAMP
OUR ADVANTAGES

Hzymes Biotech LAMP

BST DNA POLYMERASE V2

Fast polymerization

A fast polymerization is the key of Bst DNA Polymerase, the Bst DNA Polymerase V2 (HMD7003) performs almost the same speed compared against the competitor with the SARS-COV-2 N gene target. And better after 7 days thermal acceleration experiment.

BST Polymerases

Warmstart activity

To achieve higher specifificity in the LAMP reaction and even close to zero false-positive amplifification, Bst DNA Polymerase V2 (HMD7003) with a reversibly-bound aptamer could give you the warmstart function, which can block the activity under room temperature. Hzymes Bst DNA Polymerase V2 could have higher blocking efficiency at 25℃, 37℃, and 40 ℃, and can release the activity even faster at 65 ℃ than other competitors.

BSt

High Tolerance to Salt Inhibition

LAMP reactions were performed using Bst DNA Polymerase V2 (HMD7003) or a competitor Bst Polymerase (Company N) using increasing amounts of salt (potassium chloride). Bst DNA Polymerase V2 from Hzymes has better overall performance in the presence of potassium chloride.

TTR

Long-Term Stability

The activity test of Bst DNA Polymerase V2 (HMD7003) for 12 months under proper storage conditions. It has no activity change even after 12 months of storage.

TTR

RT-LAMP MASTER MIX (FLUORESCENT) - LIQUID & LYOPHILIZED

High Sensitivity and High Inhibitor Tolerance

RT-LAMP reaction is performed using a nasal swab sample spiked with SARS-CoV-2 pseudovirus(50 copies/T, 100 copies/T, and 250 copies/T) after a simple treatment with Hzymes Sample Release Buffer(HMD3504).

Highly sensitive RT-lamp

High Specificity

There is no NTC amplifification while using RT-LAMP Master Mix(Fluorescent) (HMD5207).

 

Highly specialised

Performance Consistency with Lyophilized Reagent

The data illustrate that the lyophilization process does not affect the performance of the RT-LAMP reaction. (The same template concentration: 250 copies/T, 100 copies/T, and 50 copies/T).

Ly reagent
dUTP Tolerance

Hzymes RT-LAMP Master Mix(Fluorescent) (HMD5204) performs better dUTP tolerance than company N, while using the same primer set and template.

RT-LAMP MASTER MIX (COLOMETRIC) - LIQUID & LYOPHILIZED

RT-lamp master mix
 

The nasal swab samples spiked with SARS-CoV-2 pseudovirus (50 copies/T, 100 copies/T, 250 copies/T) can be 100% detected using RT-LAMP Master Mix (Colometric)(HMD5204). The samples have been simply treated using Hzymes Sample Release Buffer (LAMP Colometric)(HMD3505)

SELECTION GUIDE FOR YOUR LAMP APPLICATION

LAMP/RT-LAMP Raw Materials

Hzymes Cat. No.

Products

Specification

HMD7002

Bst DNA polymerase V2

800U, 8000U, 80000U

HMD7002D

Bst DNA polymerase V2(High concentration)

4800U, 12000U, 120000U 

HMD7003

WarmStart Bst DNA polymerase V2 

800U, 8000U, 80000U

HMD5301

RTL reverse transcription

1500U, 15000U, 150000U

HMD5301D

RTL reverse transcription(High concentration)

9000U, 22500U, 225000U

HMD3901

RNase inhibitor

2000U, 20000U, 400000U

HMD2101

dNTP(25mM each)

0.5mL, 1mL, 5mL, 100mL

HMD2102

dNTP(10mM each)

0.5mL, 1mL, 5mL, 100mL

HMD3504

Sample release buffer

1mL, 8mL, 100mL, 1000mL

HMD3505

Sample release buffer(LAMP colometric)

1mL, 8mL, 100mL, 1000mL

LAMP/RT-LAMP related CDMO Service

Hzymes Cat. No.

Products

HCRO0011

LAMP primer design and optimization

HCRO0012

The development of lyophilization process

HCRO0013

Contract production of lyophilized reagent

LAMP/RT-LAMP Master Mix

Hzymes Cat. No.

Products

Specification

HMD5204

LAMP/RT-LAMP colometic master mix (Liquid, Lyoph-Ready)

100RXN, 1000RXN, 10000RXN

HMD5205

LAMP/RT-LAMP colometic master mix (In situ lyophilization)

100RXN, 1000RXN, 10000RXN 

HMD5206

LAMP/RT-LAMP colometic master mix (Lyophilized beads)

100RXN, 1000RXN, 10000RXN

HMD5207

LAMP/RT-LAMP flfluorescent master mix (Liquid, Lyoph-Ready)

100RXN, 1000RXN, 10000RXN

HMD5208

LAMP/RT-LAMP flfluorescent master mix (In situ lyophilization)

100RXN, 1000RXN, 10000RXN

HMD5209

LAMP/RT-LAMP flfluorescent master mix (Lyophilized beads)

100RXN, 1000RXN, 10000RXN

Leave a Comment

Your email address will not be published. Required fields are marked *