5-Me-CTP is a modified nucleoside triphosphate that replaces naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis (IVT). Methylation of the C-terminal 5 position of cytosine is a common post-transcriptional modification of mRNA. Incorporation of modified nucleosides such as 5-Me-CTP, pUTP, 2-S-UTP, etc. can increase mRNA stability and protein expression while reducing the interaction of innate immune receptors with in vitro transcribed (IVT) mRNA. Enzymatically synthesized RNA using 5-Me-CTP, pUTP, and ARCA efficiently circumvents cellular antiviral responses and reduces toxicity associated with repeated transfections of in vitro transcribed mRNA. In addition to being used to encode antigens to activate immune responses against various diseases such as COVID-19, IVT mRNA can also express heavy chain antibody heavy chain nanobodies/variable domains targeting intracellular components, labeling and manipulating antigens in vivo.
Transportation and storage method
Storage stability: -25～-15℃
Transportation stability: ≤0℃
Precautions:
- Please dissolve it on ice, and be careful to prevent RNase pollution and degradation. After use, it should be stored at -25～- 15°C.
- Avoid repeated freezing and thawing as much as possible, and do not vortex.
- For your safety and health, please wear a lab coat and disposable gloves for operation.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,