- High sensitivity,: with detection of ALP as low as 1×10⁻¹⁹ mol/L (0.01 pg);
- Fast peak intensity: reaching sustained peak intensity within 2-3 min when using ALP;
- Wide detection range: with the linear range of detection of ALP between 50-50,000 pg/mL;
- Wide linearity: linear range of detection extending over seven orders of magnitude;
- Temperature insensitivity: reaction stable between 22-35°C, no need for precise temperature control.
Use
For alkaline phosphatase (Alkaline Phosphatase, ALP) as a standard Chemiluminescent immunodetection of markers. Suitable for tube luminescence, plate luminescence, POCT chemiluminescence detection, etc. same scene.
Functional principle
HH3604 chemiluminescent substrate solution is a new generation of immunodetection chemiluminescence Optical liquid, this product is a water-based mixed solution based on APS-5 compound liquid, through the optimization of key components such as enhancers, stabilizers, and preservatives, The performance of the product has been greatly improved, and it can be used in the action of alkaline phosphatase (ALP). A chemiluminescence reaction occurs under use, and photons are rapidly released. After adding the sample The maximum luminous value can be reached in about 2 minutes, and the plateau period lasts long. This product is in the range of ALP enzyme concentration of 10-4-10-8 U, The number of photons released is proportional to the concentration of ALP in the solution, suitable for Chemiluminescence immunodetection reagent system with ALP as labeling enzyme, with It has the advantages of fast response, high sensitivity, high stability, and high signal-to-noise ratio.
Features
- The background value is low, the luminous value is high, the signal-to-noise ratio is high, and the maximum luminous value is reached The time is short, the ALP luminescence reaction enters the plateau phase within 2 min, and The luminescence value can remain stable for a long time (Fig. 1).
- High sensitivity, can detect 10-8 U or even lower concentration of ALP enzyme son.
- Wide linear range, ALP concentration is 5 orders of magnitude of 10-4-10-8U The luminescence values in the range are linearly related (Figure 2)
Figure1. Luminescence value-time of then reaction between APS-5 luminescent substrate solution and ALP enzyme kinetic curve
Figure 2.The APS-5 luminescent substrate solution has a wide linear range.
- The validity period is long, and it can be stored at 2-8℃ in the dark, and can be stored stably for more than one year.
Quality Control
Please review the Certificates of Analysis for each batch (included with the product)
Precautions
- It is normal for crystals to appear at the bottom of the refrigerated bottle of this product, and it should be balanced to room temperature. Warm (22-35℃) crystals disappear after use.
- Strictly avoid light, under any circumstances (including before use) should be avoided Exposure to strong light, short exposures cancause the background signal to show.
- Do not freeze.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
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