DNase I

Storage condition

-20℃ for storage（Avoid repeated freeze-thaw cycles）

Storage Buffer

2 mM CaCl2，10 mM Tris-HCl (pH 7.6，25℃)， 50% glycerol.

Unit Definition

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in a total reaction volume of 50 µl in 10 minutes at 37°C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Heat inactivation

75℃，10 min.

Quality Control

RNase: 2 U of DNase I with 1.6 μg MS2 RNA for 16 hours at 37 ℃ yields no degradation as determined by agarose gel electrophoresis.

E.coli DNA: 2 U of DNase I is screened for the presence of E. coli genomic DNA using TaqMan qPCR with primers specific for the E. coli 16S rRNA locus. The E. coli genomic DNA contamination is ≤1 E. coli genome.

Bacterial Endotoxin: LAL-test, according to Chinese Pharmacopoeia IV 2020 edition, gel limit test method, general rule (1143). Bacterial endotoxin content should be ≤10 EU/mg.

Notes on use

1. It is recommended to use the enzyme storage buffer for dilution.

2. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.

3. For your safety, please wear lab coat and disposable gloves during operation.