
DNase I (deoxyribonuclease I, RNase-free) is derived from a recombinant E. colistrain carrying Bovine Pancreatic DNase I. It is an endonuclease that nonspecifically cleaves DNA to release ditri- and oligonucleotide products with 5´ phosphorylated and 3´-hydroxylated ends. This enzyme was originally isolated from bovine pancreas, and the mammalian pancreas is one of the dominating sources until this day. DNase I act on various DNAs such as single and double-stranded DNA, RNA: DNA hybrids, and chromatin (the cleavage rate is affected by histones).
Product component

Storage condition
-20℃ for storage(Avoid repeated freeze-thaw cycles)
Storage Buffer
2 mM CaCl2,10 mM Tris-HCl (pH 7.6,25℃), 50% glycerol.
Unit Definition
One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in a total reaction volume of 50 µl in 10 minutes at 37°C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.
Heat inactivation
75℃,10 min.
Quality Control
RNase: 2 U of DNase I with 1.6 μg MS2 RNA for 16 hours at 37 ℃ yields no degradation as determined by agarose gel electrophoresis.
E.coli DNA: 2 U of DNase I is screened for the presence of E. coli genomic DNA using TaqMan qPCR with primers specific for the E. coli 16S rRNA locus. The E. coli genomic DNA contamination is ≤1 E. coli genome.
Bacterial Endotoxin: LAL-test, according to Chinese Pharmacopoeia IV 2020 edition, gel limit test method, general rule (1143). Bacterial endotoxin content should be ≤10 EU/mg.
Notes on use
- It is recommended to use the enzyme storage buffer for dilution.
- EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.
- For your safety, please wear lab coat and disposable gloves during operation.
- Degradation of DNA template in transcription reactions.
- Removal of contaminating genomic DNA from RNA samples.
- DNase I foot-printing: Used for Foot-printing analysis of DNA-protein interactions.
- Nick Translation: Used in conjunction with DNA Polymerase I for nick translation.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
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