FluoS Loop-Mediated Amplification​

This kit is supplied with high concentration BstL DNA Polymerase, thermostable Reverse Transcriptase and the 5×buffer, which contiansMg2+, dNTP. A fluorescent dye is also supplied to enable real-time fluorescence measurement.

Product components

Usage

For DNA or RNA isothermal amplification using Probe or fluorescent dye.

Transportation and Storage Conditions

Transported with ice bags or dry ice, stored at -20℃. Avoid repeated freezing and thawing, the product is valid for 12 months.

Protocol

1 Using Probe

1.1 Thaw buffer to be used at room temperature. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.

1.2. Prepare reaction mix as described below

10×Primer Mix: FIP/BIP 16μM，LoopF/B 4 μM，F3/B3 2 μM. Probe concentration may be optimized according to specific situations.

1.3. Reaction program

*Collect fluorescent signal.

2 Using fluorescent dye

1.1 Thaw buffer to be used at room temperature. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.

1.2. Prepare reaction mix as described below.

10×Primer Mix: FIP/BIP 16μM，LoopF/B 4 μM，F3/B3 2 μM.

1.3. Reaction program

Collect fluorescent signal.

Notices

Salt may appear in the bottom of the tube, vortex briefly or invert tubes several times to mix thoroughly The reaction temperature can be optimized between 62°C and 68°C according to the primer conditions.