Hi-yield T7 in vitro transcription reagent ( low dsRNA )

Product Overview:

The T7 In Vitro Transcription Kit (Low dsRNA) has been optimized for transcription reactions using a mutant variant of T7 RNA polymerase. It allows for in vitro transcription using linear double-stranded DNA templates containing T7 promoter sequences and NTPs as substrates to transcribe downstream DNA sequences. This kit enables customers to easily and quickly obtain a large quantity of RNA products, with lower levels of dsRNA in the resulting products. This kit can also be used with modified nucleosides as substrates to produce biotinylated, dye-labeled, or radiolabeled RNA, as well as capped RNA with cap-like structures. The kit includes the commonly used modified nucleoside N1-Me-pUTP for transcription needs.

With a single reaction, this kit can transcribe and yield 150~200 μg of RNA products, suitable for milligram-scale RNA production. The transcribed RNA can be used in various applications, including RNA structure and function studies, RNase protection, probe hybridization, anti-sense RNA, and RNAi. It can also be modified using the Vaccinia Capping System (HBP000606) and 2′-O-methyltransferase (HBP000701) for capping, or Poly(A) Polymerase (HBP000801) for polyadenylation, to generate mRNA for downstream applications like in vitro translation and transfection.

Transport and Storage:

Transport at temperatures at or below 0°C, and store at temperatures ranging from -25°C to -15°C.

Catalog number

HBP001511