Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP)

Product Overview:

The High-Yield T7 In Vitro Transcription Kit is optimized for transcription reactions using T7 RNA polymerase. It allows for the efficient in vitro transcription of linear double-stranded DNA templates containing T7 promoter sequences with NTPs as substrates. This enables customers to quickly and easily obtain a large quantity of RNA products. This kit can also be used with modified nucleosides to produce biotinylated, dye-labeled, or radiolabeled RNA. It can be used with cap-structured or cap-like-structured substrates to obtain capped RNA. The kit includes the commonly used modified nucleoside N1-Me-pUTP for transcription needs.

With a single reaction, this kit can transcribe and yield 150~200 μg of RNA products, making it suitable for milligram-scale RNA production. The transcribed RNA can be used in various applications such as RNA structure and function studies, RNase protection, probe hybridization, anti-sense RNA, RNAi, and can also be modified using the Vaccinia Capping System (HBP000606) and 2′-O-methyltransferase.

Transport and Storage:

Transport at temperatures at or below 0°C, and store at temperatures ranging from -25°C to -15°C.

Catalog Number

HBP001505