Hot Start PCR Master Mix (Chemically modified)
Taq DNA polymerase is a heat-resistant DNA polymerase derived from Thermus aquaticus YT-1. The enzyme has a polymerase activity of 5’→ 3’and a specificity of 5’→ 3 for the double-strand ‘Exonuclease activity. The enzyme uses a chemical modification group to block the enzyme activity at low temperature and room temperature. After thermal activation at 95°C, the chemically modified group falls off to release the enzyme activity. Before the amplification system is formulated to PCR amplification, non-specific amplification due to primer mismatch will not occur, which minimizes the formation of non-specific amplification and primer dimers, and improves the sensitivity and specificity of amplification. This product uses a specially optimized reaction buffer and hot-start Taq DNA polymerase (chemically modified) to make a premixed reagent, which maximizes the stability of the enzyme during storage. Only the primer template and water are added before configuring the system. , Greatly simplifies the preparation steps.
This product has strong versatility and is suitable for end-point PCR, dye and probe qPCR.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,