Homocysteine Assay kit (Cystathionine Assay)​

Homocysteine Kit (Enzymatic Cycling Method)

Sample Requirements

1. Serum, heparin or EDTA plasma, and urine are suitable for samples. Whole blood, hemolysis is not recommended for use as a sample. Freshly drawn serum is the preferred specimen.

2. Stability: Serum/plasma: 5 days at 2–8℃;

Calibrator Preparation

Liquid calibrator and Control can be used when opened.

Control Preparation

Liquid calibrator and Control can be used when opened.

Method

1. Reagent preparation：Liquid reagent can be used when opened.

Calibration

It is recommended to use the Calibrator from Hzymes and distilled/deionized water for two-point calibration.

Calibration frequency: After reagent lot changed; As required following quality control procedures; The value of control change significantly; the biochemical analyzer has undergone extensive maintenance.

Quality control

At least two levels of control material should be analyzed with each batch of samples.Each laboratory should establish its own internal quality control scheme and procedures for corrective action if controls do not recover within the acceptable tolerances.

Reference Intervals

[3] Each laboratory should establish its own reference intervals based upon its patient population. The reference intervals measured at 37 °C listed below were taken from literature.

Serum / Plasma: 4.0～15.4μmol／L

Interferences/Specificity

The following substances were tested for interference with this methodology. Criterion: Recovery within ±10 % of initial value.

In vitro test for the quantitative determination of HCY concentration in serum or plasma on photometric systems. Homocysteine (Hcy) is a thiol-containing amino acid produced by the intracellular demethylation of methionine. Total homocysteine (tHcy) represents the sum of all forms of Hcy including forms of oxidized, protein bound and free. Elevated level of tHcy has emerged as an important risk factor in the assessment of cardiovascular disease. Excess Hcy in the blood stream may cause injures to arterial vessels due to its irritant nature, and result in inflammation and plaque formation, which may eventually cause blockage of blood flow to the heart

Anti-interference Performance:

Hemoglobin ≤500 mg/dL             Triglyceride ≤1250 mg/dL

Vitamin C ≤20 mg/dL                      Bilirubin ≤50 mg/dL