Hot start Taq DNA polymerase Super Mix (chemical modification)
Taq DNA polymerase is a heat-resistant DNA polymerase derived from Thermus aquaticusYT-1. The enzyme has a polymerase activity of 5’→ 3’and a specificity of 5’→ 3 for the double-strand ‘Exonuclease activity. The enzyme uses a chemical modification group to block the enzyme activity at low temperature and room temperature. After thermal activation at 95°C, the chemically modified group falls off to release the enzyme activity. Before the amplification system is formulated to PCR amplification, non-specific amplification due to primer mismatch will not occur, which minimizes the formation of non-specific amplification and primer dimers, and improves the sensitivity and specificity of amplification. This product uses a specially optimized reaction buffer and hot-start Taq DNA polymerase (chemically modified) to make a premixed reagent, which maximizes the stability of the enzyme during storage. Only the primer template and water are added before configuring the system. , Greatly simplifies the preparation steps. This product has strong versatility and is suitable for end-point PCR, dye and probe qPCR.
single specific 5 kb band.
- Exonuclease detection: add 1μM 5′-FAM-labeled oligonucleotide to 20μl reaction system, and incubate at 37°C for 30min without degradation signal.
- Heat inactivation: None detected
- Storage stability: Repeated freezing and thawing of this reagent 50 times, the amplification performance will not decrease.
b: The primer volume is the recommended dosage, which can be optimized according to the actual situation.
a: The pre-denaturation time for common templates is 1 min, the recommended time for difficult templates is 2-3 min, and the colony PCR pre-denaturation time is recommended to be 5 min.
b: The recommended annealing time is 15-60s. The annealing temperature is generally determined at 45-68℃ according to the Tm of the primer, and it is generally set to be 3-5℃ lower than the Tm of the primer.