Hot Start Taq DNA Polymerase Super mix (multiplex)
- Becomes activated rapidly after heat treatment at 95°C for 2 min
- Maintains a high degree of sensitivity at the minimum detectable concentration of 0.1 pg
- Supported by the contamination-resistant Master Mix compatible with UDG/dUTP
- Compatible with multiple PCR systems
- Suitable for Taqman/SYBR qPCR assays
- Resistant to 10% whole-blood interferon release.
Hot Start Taq DNA Polymerase is a heat-resistant DNA polymerase derived from Thermus aquaticus YT-1. The enzyme has 5’→3′ polymerase activity and double-strand specificity 5 ‘→ 3’exonuclease activity. The enzyme uses antibody modification to block low temperature and room temperature enzyme activity. After thermal activation at 95°C, the antibody falls off to release the enzyme activity. Before the amplification system is formulated to PCR amplification, non-specific amplification due to primer mismatch will not occur, which minimizes the formation of non specific amplification and primer dimers, and improves the sensitivity and specificity of amplification. The product uses a specially optimized reaction buffer, and Hzymes Accu AM Hot Start Taq DNA Polymerase to make a premixed reagent, which maximizes the stability of the enzyme during storage. Only the primer template and water are added before configuring the system, which greatly simplifies The preparation steps.
Product Components
Transportation and storage methods
≤0℃ for transportation; -20℃ for storage.
Unit definition
Using activated salmon sperm DNA as a template/primer, the activity of ingesting 10 nmol of total nucleotides as acid insoluble at 74°C in 30 min is defined as 1 activity unit (U).
Quality Control
- Endonuclease residue detection: 4 μg pUC19 DNA is added to this reagent and incubated at 37 ℃ for 4 h, the electrophoresis band of the DNA does not change.
- 5 kb λDNA amplification detection: After 25 cycles of amplification with 5 ng λDNA as a template, agarose gel electrophoresis shows single specific 5 kb band.
- Exonuclease detection: add 1μM 5′-FAM labeled oligonucleotide to 20μl reaction system, and incubate at 37°C for 30min without degradation signal.
- Heat inactivation: None detected
- Storage stability: Repeated freezing and thawing of this reagent 50 times, the amplification performance will not decrease.
Reaction System
a:
a: The pre-denaturation time for common templates is 1 min, the recommended time for difficult templates is 2-3 min, and the colony PCR pre-denaturation time is recommended to be 5 min.
b: The recommended annealing time is 15-60s. The annealing temperature is generally determined at 45-68℃ according to the Tm of the primer, and it is generally set to be 3-5℃ lower than the Tm of the primer.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
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