Recombinant trypsin digestion

Trypsin is a serine protease that cleaves peptide bonds on the C-terminal side of lysine and arginine, hydrolyzes intercellular proteins, and disrupts intercellular connections, thereby dispersing tissue or adherent cells into individual cells. The recombinant trypsin digestion solution of Hzymes biotech is a high-purity recombinant enzyme fermented by micro-organisms. It does not contain animal origin. It can replace porcine or bovine trypsin and it is used for cells in the fields of immune cell therapy, vaccines, drug screening, antibodies, etc. digestive process. This product contains EDTA and does not contain phenol red.

Product properties:  

Due to its high purity, only a single enzyme acts, the dissociation specificity is stronger, and the toxicity is lower. This mechanism also reduces the dissociative damage of various proteases in trypsin and other extraction reagents. 

No animal origin: 

No animal origin raw materials used in the production process, there is no risk of potential uncertainties such as exogenous virus contamination, and it complies with drug management regulations. 

Good stability: 

Low temperature conditions, 2～8℃/-20～-5℃ Can be stored in the dark for 12 months 

Easy to use: 

This product is a sterile preparation and can replace trypsin in experimental protocols, dilution can reduce activity

Transportation and storage method Storage conditions: 

2-8℃, protected from light. Avoid repeated freezing and thawing Expiration date: 12 months from date of manufacture. Transport conditions: 2-8℃, dark transport.

Instructions for use

Strongly adherent adherent cell line. 

1. Preheat the digestion solution and culture solution at 37°C before use. Note: Recombinant trypsin digestion solution can be used to digest a variety of cells at room temperature. 
2. Aspirate the used medium and discard. 
3. Wash cells with 5 mL of calcium- and magnesium-free DPBS buffer to remove residual medium, aspirate medium and discard. 
4. Add an appropriate volume of recombinant trypsin digestion solution, as far as covering the cells. 
5. Incubate the cells at 37°C and observe them under a microscope during the incubation period, until the cells shrink significantly and the bottom of the culture vessel is visually observed to see that the morphology of the cells has changed significantly, or tap the culture flask to find that the cells are in a free state. 
6. Add 5–10 mL of prewarmed complete medium to the flask. Tilt in all directions to ensure that all cells are suspended, and transfer the cell suspension to a 15 mL centrifuge tube. 
7. Centrifuge at 100 × g for 5-10 minutes. 
8. Discard the supernatant and resuspend the cell pellet in 2–5 mL of prewarmed medium. 
9. Determine viable cell density and percent viable using an automated cell counter. 
10. Follow the routine protocol for subsequent experiments, depending on your cell type. 

Precautions

For your safety and health, please wear a lab coat and gloves. This product cannot used on human body. Please operate in a sterile environment