Recombinant Trypsin

Storage Condition

Stability of storage: Recombinant trypsin lyophilized power should be stored under 2-8℃. It is stable within 24 months. After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing. Stability of transport: The product is stable by ice bag insulation transport.

Unit Definition

25℃, pH 7.6, 3.2ml reaction solution(1cm light path), one trypsin unit (U) was defined as an increase of 0.003 in the absorption value at 253nm by enzymatic hydrolysis of BAEE per minute.

Quality Control

• Activity: Determination the activity of trypsin by Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition).

• E. Coli host cell protein residue: ELISA Kit. Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition) –E. Coli host Cell protein residue (3412).

• Protein purity：Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition)- General Rules for HPLC Method (0512).

• Bioburden: Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition)- General Rules for Sterility Test (1101).

• Host Cell DNA residue: Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition) Host Cell DNA residues (3407).

• Endotoxin: Pharmacopoeia of the People’s Republic of China Volume 4 (2020 Edition)- General Rules for LAL-Test (1143).

Product Instruction

1. Solution preparation: Take an appropriate

2. amount of trypsin according to the protein content (calculated by power activity and protein activity) of COA, and add HBSS equilibrationsolution (or other buffer suitable for cell digestion. If necessary, add EDTA to a final concentration of 0-1mM, it is best not to exceed 2mM). The recommended trypsin concentration is about 0.1- 0.3mg/mL (concentration adjusted according to different cells), and gently mixed; this step can be operated at room temperature.

   2. Use a 0.22μm filter to filter the trypsin solution and transfer it to a sterile container; this step can be operated at room temperature.
   3. The filtered trypsin solution should be used directly as required on the day (for example, adding 1mL to T25 bottles, digesting cells at 37°C), and the liquid can be stored at 2-8°C for 1-2 weeks.
   4. Long-term storage: If the prepared recombinant cell digestion solution needs to be stored for a long time, it can be stored stably at – 20°C for 12 months.

   Product Usage

   For cell culture

   • Tissue block digestion, primary cell acquisition.
   • Passage digestion of adherent cells.
   • Cell culture by microcarrier method.
   • Gently digesting stem cells.
   • Immune cell therapy, etc.

   For recombinant protein:

   • Recombinant insulin production.
   • Protein sequencing, peptide mapping
   • Specific proteolytic processes such as proteomics research.

     Product Advantage

     • No animal origin: recombinant production, no exogenous virus contamination, production process does not use any animal raw materials.
     • High purity: high β-trypsin ratio and protein purity.
     • Better process control: low content of E. Coli host Cell protein, host Cell DNA, bioburden, metal ions and endotoxin.
     • Stable quality: stable batch production can ensure continuous batch production; no difference between batch and batch.

     Precautions

     • High concentration of inorganic salts have an obvious effect on the activity.
     • Avoid contact with other protein products.
     • For your safety and health, please wear lab coat and gloves for operating.