Product Information
Product Description
Murine RNase inhibitor is a recombinant murine RNase inhibitor expressed and purified from E.coli. It binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the three enzymes and protecting RNA from degradation. However, It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. Murine RNase inhibitor was tested by RT-PCR, RT-qPCR and IVT mRNA, and was compatible with various commercial Reverse transcriptases, DNA polymerases and RNA polymerases. Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That making it stable at low concentrations of DTT (less than 1 mM). This feature makes it suitable for use in reactions where high concentrations of DTT is adverse to the reaction (eg., Real-time RT-PCR).
Storage Temperature
Store at -20 ℃, valid for 2 years
Storage Buffer
50 mM KCl, 20 mM HEPES-KOH (pH 7.6, 25 ℃), 8 mM DTT and 50% glycerol.
Unit Definition
The amount of murine RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50% was defined as one unit (U).
Molecular Weight of Protein
The molecular weight of murine RNase inhibitor is 50 kDa.
Quality Control
Exonuclease Activity: 40 U of murine RNase inhibitor with 1 μg λ-Hind III digest DNA at 37 ℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Endonuclease Activity: 40 U of murine RNase inhibitor with 1 μg λDNA at 37 ℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Nicking Activity: 40 U of murine RNase inhibitor with 1 μg pBR322 at 37 ℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.
RNase Activity: 40 U of murine RNase inhibitor with 1.6 μg MS2 RNA for 4 h at 37 ℃ yields no degradation as determined by agarose gel electrophoresis.
E.coli DNA: 40 U of murine RNase inhibitor is screened for the presence of E. coli genomic DNA using TaqMan qPCR with primers specific for the E. coli 16S rRNA locus. The E. coli genomic DNA contamination is ≤ 0.1 pg/ 40 U.
Endotoxin: LAL-Test, General rule (1143) of Gel Limit Test in Chinese Pharmacopoeia (2020 edition volume Ⅳ), the bacterial endotoxin content is ≤ 10 EU/mg.
Product Application
This product can be widely used in any experiment where RNase interference is possible to avoid RNA degradation, such as:
Usage Note
+86-15821125706 info@hzymes.com Marketing Center NO. 1588, Huhang Road, Office 701, Shanghai , Shanghai CN