This kit is supplied with BstL DNA Polymerase, thermostable Reverse Transcriptase and the buffer, which contians Mg2+, dNTP and visible dye. This kit provides a fast, clear visual detection of amplification, which negative reaction is indicated in red and positive reaction is indicated by a change to yellow
Product components
Usage
For DNA or RNA isothermal amplification.
Transportation and Storage
Conditions
Transported with icze bags, stored at -25 ～ -15℃. Avoid repeated freezing and thawing, the product is valid for 12 months.
Protocol
- Thaw buffer to be used at room temperature and placed on ice. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.
- Prepare reaction mix as described below.
10×Primer Mix: FIP/BIP 16μM，LoopF/B 4 μM，F3/B3 2 μM.
- Incubate at 65°C for 30 – 45 minutes. Return reactions to 65℃ for an additional 10 minutes if color is not yellow.
- Examine colour by eye, positive reactions will have turned yellow while negative controls should remain red.
Notices
- Buffer needs to be fully melted at room temperature and stored at – 25~- 15 ℃ after use.
- Reagents after subpackage shall not be exposed to air for a long time.
- The red yellow discoloration reaction depends on the pH change of the reaction system. Please do not use the nucleic acid preservation solution containing Tris. It is recommended to use the nucleic acid preserved by ddH2Ons.
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
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