T4 UvsY Protein

T4 UvsY Protein is a Phage T4 recombinant regulatory protein that promotes homologous recombination performed by UvsX. When UvsX looks for homologous sequences, it needs to compete for binding sites with single-stranded DNA-binding proteins prebound to single-stranded DNA, and UvsY protein promotes this competition, which helps UvsX to bind to single-stranded DNA. T4 UvsY Protein enhances the activity of the DNA-dependent ATPase of UvsX Protein, reducing the minimum concentration required for the activity, thus promoting chain replacement. UvsY promotes the invasion of UvsX recombinase into ssDNA-single-stranded binding protein complex, resulting in the release of single-stranded binding protein, and thus the binding of UvsX to single-stranded DNA.

Storage Temperature

Store at -20 ℃, valid for one year (Avoid repeated freeze-thaw cycles).

Protein Concentration

The concentration of T4 UvsY Protein is 1 mg/mL±5% as determined by UV absorption at 280 nm.

Storage Buffer

20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerol.

Heat Inactivation

60℃ for 10 minutes.

Quality Control

Exonuclease Activity: 1 μg of T4 UvsY Protein with 1 μg λ-Hind III digest DNA at 37℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.

Endonuclease Activity: 1 μg of T4 UvsY Protein with 1 μg λDNA at 37℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.

Nicking Activity: 1 μg of T4 UvsY Protein with 1 μg pBR322 at 37℃ for 16 hours yields no degradation as determined by agarose gel electrophoresis.

RNase Activity: 1 μg of T4 UvsY Protein with 1.6 μg MS2 RNA for 16 hours at 37℃ yields no degradation as determined by agarose gel electrophoresis.

E. coli DNA: 1 μg of T4 UvsY Protein is screened for the presence of E. coli genomic DNA using TaqMan qPCR with primers specific for the E. coli 16S rRNA locus. The E. coli genomic DNA contamination is ≤1 E. coli genome.

Notes On Use

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.

1. DNA isothermal amplification.
2. RPA amplification.