Advancements in Chemiluminescence Immunoassays: AMPPD vs. APS-5 Chemiluminescence Substrates Chemiluminescence immunoassays (CLIA) have revolutionized the field of diagnostics and analytical chemistry due to their exceptional sensitivity, wide dynamic range, and versatility. CLIA relies on the emission of light resulting from a chemical reaction between a chemiluminescent substrate and an enzyme-labeled antibody or antigen. Two widely …