Fluorescence vs. Chemiluminescence: A Comparative Analysis for Quantitative Enzyme Assays using Hzymes APS-5 and AMPPD In the realm of analytical chemistry and biochemistry, the choice of detection method plays a pivotal role in the accuracy and sensitivity of quantitative assays. Two commonly employed techniques are fluorescence and chemiluminescence, each offering unique advantages and applications. This …
Chemiluminescence immunoassays
Advancements in Chemiluminescence Immunoassays: AMPPD vs. APS-5 Chemiluminescence Substrates Chemiluminescence immunoassays (CLIA) have revolutionized the field of diagnostics and analytical chemistry due to their exceptional sensitivity, wide dynamic range, and versatility. CLIA relies on the emission of light resulting from a chemical reaction between a chemiluminescent substrate and an enzyme-labeled antibody or antigen. Two widely …