
UNIT DEFINITION
One unit (U) is defined as the amount of enzyme which consumes 1 μmol of uric acid per min under the conditions described below.
REAGENTS PREPARATION
Reagent I: 0.001% uric acid.
Enzyme diluent: Add 3.09 g boric acid, 0.37 g EDTA-Na2·2H2O and 0.01 g Triton X-100 in 800 ml pure water, adjust to pH 8.5 by NaOH and set to 1000 mL by pure water.
Sample: The enzyme was diluted to 0.05-0.1 U/mL with enzyme diluent.
PROCEDURE
- Preincubate the Reagent I at 37 ℃.
- Add 2 mL of Reagent I, 0.5 mL of pure water, and then add the diluted enzyme to a cuvette.
- Record the ΔAs at 290 nm in 1 minute in a spectrophotometer thermo stated at 37 ℃.
At the same time, measure the blank rate ΔAb by using the same method as the test except that the enzyme diluent is added instead of the enzyme solution.
∆A = ∆As – ∆Ab

3.0: Total volume (mL)
0.5: enzyme volume (mL)
1.0: Light path length (cm)
df: Dilution factor
C: Enzyme concentration (mg/mL)
12.2: Millimolar extinction coefficient of uric acid under 290 nm (cm2/μmol)
REFERENCES
- Bongaerts, G. P. A., Uitzerter, J., Brouns, R. and Vogeis, G. D. (1978) Biochim. Biophys. Acta, 527, 348–358.
- Bongaerts, G. P. A. and Vogeis, G. D. (1976)
- Bacteriol., 125, 689–697.
This enzyme is useful for enzymatic determination of uric acid in clinical analysis

The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
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