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UltraNuclease is a genetically engineered broad-spectrum nuclease originating from Serratia Marcescens. It can degrade all forms of DNA and RNA, including single-stranded, double-stranded, linear, natural and denatured nucleic acids, and digest them into 5′-oligonucleotide monophosphate of 3-5 base in length with no base-specific recognition. Therefore, it is the most ideal enzyme preparation for removing nucleic acid.
Principle of the Procedure
This UltraNuclease ELISA kit is a sandwich ELISA to be performed in a microplate format. Sample potentially containing endonuclease are incubated in microtiter plate wells which have been pre-coated with an affinity purified anti-endonuclease capture antibody. After incubation and a washing step in which unbound components are removed, an enzyme-conjugated, anti-endonuclease detector antibody is added. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-endonuclease-enzyme labeled antibody. After a final washing step, a substrate solution is added to the wells and reacted, resulting in color development. The optical density is measured photometrically and is proportional to the analyte concentration present in the wells. The endonuclease concentration in unknown samples can be calculated based on the corresponding standard curve.
Kit Component
Storage and Stability
Equipment required
Reagent Preparation
Assay Procedure
Assay Flowchart
Calculation of Result
Performance Characteristics
Hzymes Biotechnology Co., Ltd. Endonuclease from other suppliers may show a different reactivity with regard to sensitivity and accuracy, therefore the compatibility of the kit calibration to the individual endonuclease product must be verified.
Precautions
The Certificate Of Analysis (COA) & Material Safety Data Sheet (MSDS) Is A Signed Document That Includes The Storage Temperature,
Feel free to contact us. We are providing the best product & service for customers worldwide…
+86-15821125706 info@hzymes.com Marketing Center NO. 1588, Huhang Road, Office 701, Shanghai , Shanghai CN